Thioacidolysis-Based Lignin Composition Analysis

Ground leaf tissues were washed serially with water, 80% ethanol, and acetone, and freeze dried. The washed leaf tissues (~3 mg) were then subjected to analytical thioacidolysis according to the method described by Yamamura et al. (2012). The released lignin monomers were derivatized with N,O-bis(trimethylsilyl)acetamide and quantified by gas chromatography-mass spectrometry, using 4,4′-ethylenebisphenol as an internal standard (Yue et al., 2012). Experiments were repeated two times with similar results.