Chemicals and antibodies

In this study, the following antibodies and chemicals were used, including fenbendazole (MedChemExpress, cat# HY-B0413). The antibodies against phospho-Akt(p-Akt) (S473) (Cell Signaling Technology; cat# 9271), Akt (Cell Signaling Technology; cat # 9272), PLC-γ1(Cell Signaling Technology, cat# 2822S), p-PLC-γ1(Ser1248) (Cell Signaling Technology, cat# 8713), GAPDH (Cell Signaling Technology, cat# 2118), and β-Actin (Cell Signaling Technology; cat# 4970) were used. Goat anti-BoHV-1 serum was purchased from VMDR Inc, Gandhinagar, India [20PAB-IBR]. HRP-(horseradish peroxidase-) conjugated goat anti-mouse IgG (Cell Signaling Technology, cat# 7076), HRP-goat anti-rabbit IgG (Cell Signaling Technology, cat# 7074), and HRP-donkey anti-goat IgG H&L (Abcam, ca# ab97110).

BoHV-1 VP16 antibody (1:2,000) was kindly provided by Prof. Vikram Misra at the University of Saskatchewan [20].