Nonphotochemical quenching measurements

LG Laura Girolomoni
FB Francesco Bellamoli
GV Gabriel de la Cruz Valbuena
FP Federico Perozeni
CD Cosimo D'Andrea
GC Giulio Cerullo
SC Stefano Cazzaniga
MB Matteo Ballottari
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NPQ was calculated as (Fm-Fm)/Fm (Bilger & Björkman, 1990) using a Dual PAM‐101 (Waltz, Effeltrich, Germany). F m is the maximum Chl fluorescence emitted by dark‐adapted cells after 2 min treatment with a far‐red LED, Fm is the maximum fluorescence measured upon exposure to actinic light or during dark recovery. Far‐red light was turned on also during dark recovery. A 5000 µmol m−2 s−1 saturation pulse was used to induce F m and Fm while the intensity of the actinic lights used were reported in the results section.

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