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AN3CA and Ishikawa cells were planted into 96-well plates, and cultured with 20 μL MTT (0.5mg/mL) at 24, 48, 72 h. Then, the precipitated formazan was dissolved in 150 µL of dimethyl sulfoxide (DMSO). The OD values at 450 nm (A450) were assessed using a microplate reader (Bio-Rad, CA, USA).
Copyright and License information: ©2020 Zhang et al.
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