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2.4. Chromatin Immunoprecipitation (ChIP) Assay
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Cells were crosslinked with 1% formaldehyde and then lysed in SDS lysis buffer (50 mM Tris-HCl, pH 8.0, 1% SDS, 10 mM EDTA, and protease inhibitors). The ChIP procedure was performed as previously described [24]. Primer sequences are listed in Table 2.
Primer sets for ChIP-qPCR.
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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