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To measure CORT levels in experiment 1, animals were sacrificed by decapitation and trunk blood was collected in a cup containing ethylenediaminetetraacetic (9 g/100 ml) acid (EDTA; Hagewoud, Whitcomb, et al., 2010; van der Borght et al., 2006). Subsequently, the samples were centrifuged at 2,600 g at 4°C and the supernatant was collected in polycarbonate cups. Plasma CORT levels were measured by a double antibody radioimmunoassay method for rodents, using an immuChem™ kit (MP Biomedicals LLC). CORT levels of samples were measured in duplicate.
Copyright and License information: ©2019 The Authors. Journal of Sleep Research published by John Wiley & Sons Ltd on behalf of European Sleep Research Society
This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
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