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Markers of microbial translocation, and immune mediators in plasma and cell culture

KS Kasha P. Singh
JZ Jennifer M. Zerbato
WZ Wei Zhao
SB Sabine Braat
CD Claire Deleage
GT G. Surekha Tennakoon
HM Hugh Mason
AD Ashanti Dantanarayana
AR Ajantha Rhodes
JR Jake W. Rhodes
JT Joe Torresi
AH Andrew N. Harman
PR Peter A. Revill
MC Megan Crane
JE Jacob D. Estes
AA Anchalee Avihingsanon
SL Sharon R. Lewin
JA Jennifer Audsley
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Lipopolysaccharide (LPS), CXCL10 and CCL2 were quantified as previously described [9]. Plasma soluble (s) CD14 and High mobility group box 1 (HMGB1), a marker of cell damage and death [31] were measured by ELISA in duplicate according to the manufacturer’s instructions (R&D Systems, McKinley Place, MN and IBL International GMBH, Hamburg, Germany respectively). For in vitro experiments, CXCL10 was quantified by single-analyte ELISA according to the manufacturer’s instructions (Human CXCL10/IP-10 ELISA MAX Deluxe Set, Biolegend, San Diego, CA).

Copyright and License information:  ©2020 Singh et al
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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