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Construction of mapping population and BSA

JW Junqing Wu
JC Jie Cheng
CX Chunmiao Xu
SQ Shilian Qi
WS Wenru Sun
SW Shuang Wu
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To map the candidate gene responsible for rf, the mutant was crossed with WT, and the F1 plants were self-fertilized to generate the F2 population. The segregation ratio of F2 was analyzed by the chi-square test. Genomic DNA was extracted from 55 individuals with mutant phenotypes in the F2 population and 55 WT individuals by a modified CTAB method.

The qualified DNA was mixed to construct mutant-like bulk and WT bulk. Both the sequencing parameters and analysis methods were based on Chang et al.’s study53. The ORF with an ~100% SNP index in the mutant-like bulk was used for mutation identification and complementary verification.

Copyright and License information: The Author(s) ©2020
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