2.3. Induction of ER stress by tunicamycin

Tunicamycin from Streptomyces sp (Sigma) was dissolved in dimethyl sulphoxide (DMSO) and diluted in sterile 150 mM dextrose to obtain a tunicamycin concentration of 10 µg/µl. Mice (20‐25 g) were randomized into 3 groups, in which they either received a burn injury, or injected intraperitoneally with one dose of tunicamycin solution (1 µg/g body mass) either 24 hours pre‐burn or 24 hours post‐burn and assessed for survival. As controls, mice were injected intraperitoneally with control buffer (150 mM dextrose containing 1% DMSO).