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Alpha-synuclein western blots
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Briefly, after extracted and normalized the total protein from the hippocampus, the samples without boiling were separated by 4–20% non-denaturing polyacrylamide-gradient gel electrophoresis (Thermo Fisher Scientific, USA). Then, the samples were transferred to PVDF membranes and incubated with the alpha-Synuclein antibody (1:500, Invitrogen, Cat. no. #MA5-12272). After 24 h, the membranes were incubated with a matched secondary antibody for 2 h. The results were detected using ECL and analyzed using image analysis software (ImageJ 1.42q, USA).
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