Thioflavine T fluorescence assay

AS Alexey K. Surin
SG Sergei Yu. Grishin
OG Oxana V. Galzitskaya
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Fibrils growth was monitored on the basis of thioflavine T (ThT) fluorescence. The kinetics of aggregation of insulin and its analogues was studied by us using the method of fluorescence spectroscopy as described previously [47]. Each assay was done in triplicate. The plot of each kinetic was obtained as the average of multiple results measured at different time intervals.

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