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Tumors were established in 8 male athymic nude mice per group (Harlan, Indianapolis) at 6 weeks old by injecting 1 × 106 DU145 (WT, C81S, ΔKTEL) cells in 100 µl of matrigel subcutaneously. Tumor growth was measured by using calipers to measure dimensions orthogonally. Volumes were calculated by the general equation of (SxL)/2, where S and L represent the small and large dimensions of the tumor lesions. No animals were excluded.
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