4.2. Yeast One-Hybrid Assay

In yeast one-hybrid assay, the PGADT7:VirD5 and pAbAi reporter under the control of D5RF.1 promoter (Supplementary Table S2) were co-transferred into yeast strain Y1H Gold. The transformed yeast cells were first spotted on SD minus Uracil and Leucine (SD/-Ura-Leu) medium, and then the cultured yeast colonies were resuspended in sterile ddH₂O, adjusted to an OD₆₀₀ of 0.8, and transferred onto SD/-Leu-Ura minimal medium and SD/-Leu-Ura minimal medium with 500 ng.mL⁻¹ Aureobasidin A for culture. Qualitative colony-lift filter assays was performed according to the manufacturer’s protocols (Clontech, Protocol No. PT3024-1).