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Actin polymerization assays were performed at 22°C using a PTI Fluorometer (Photon Technology International) as previously described (Chen et al., 2014a). Reactions contained 4 μM actin (5% pyrene labeled), 10 nM Arp2/3 complex, 100 nM WRC217VCA (Figure 1—figure supplement 1c; consisting of Sra1, Nap1, HSPC300, Abi2(1-158) and WAVE1(1–217)-(GGS)6-(485-559)) or WRC230VCA (Figure 6; consisting of Sra1, Nap1, HSPC300, Abi2(1-158) and WAVE1(1–230)-(GGS)6-(485-559)) or VCA, and various amounts of Rac1 Q61L loaded with GMPPNP.
Copyright and License information: ©2017, Chen et al
This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
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