qPCR data from faeces and ileum mucosa samples were log10 transformed (after adding 1 to avoid zeros) and expressed in log10 pg DNA/μl. The average values were plotted with the 95% confidence interval where no overlap indicates statistical significance.
In addition, the AUC was calculated by the linear trapezoidal rule as a measure of total shedding over time. The AUC of the qPCR data of faeces, the faeces qPCR data on day 21 and the qPCR data of the ileum mucosa on day 21 were statistically analysed by Analysis of Variance (ANOVA) using Tukey’s post-hoc test to compare groups.
Copyright and License information: The Author(s) ©2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this
article to respond.
Ask a
question
Post a Question
