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Evaluation of tubulointerstitial fibrosis

HS Hayato Sasaki
JK Junpei Kimura
KN Ken-Ichi Nagasaki
KM Kiyoma Marusugi
TA Takashi Agui
NS Nobuya Sasaki
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Formalin-fixed and paraffin-embedded kidney blocks were sectioned at a thickness of 3 μm, and processed for Masson’s trichrome (MT) staining. Five random renal cortex fields for each sample were chosen, and the aniline blue (AB)-stained area, representing interstitial collagen spread, was measured using ImageJ software.

Copyright and License information: The Author(s). ©2016
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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