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Size-exclusion chromatography analysis of aggregation
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60 µg of each sample was separated isocratically at ambient temperature (~22° C) using a sodium phosphate mobile phase (100 mM sodium phosphate, 250 mM sodium chloride, pH 6.8) on a 4.6 × 150 mm, 1.7 µm Waters Acquity BEH200 SEC column. The column flow rate was 0.4 mL/min and detection was monitored by A280 with a method run time of 6 minutes.
Copyright and License information: ©2016 Just Biotherapeutics
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