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2.5. Transwell assays

WS Wenchao Shao
XL Xiaoguang Li
JP Jiangtong Peng
SF Siyuan Fan
ML Minglu Liang
KH Kai Huang
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Transwell assays were used to determine cell migration. After pretreatment of apatinib for 4 hours, VSMCs were seeded into the upper chambers, 500 μL DMEM with 10% FBS and PDGF‐BB (30 ng/mL) was placed into the lower chamber. 12 hours later, the migrated cells in the lower chamber were fixed with 4% formaldehyde for 20 minutes and then stained with 0.1% crystal violet for another 20 minutes. The migrated cells were photographed using Olympus cellSens Entry.

Copyright and License information:  ©2020 The Authors. published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd.
This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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