Rotarod test

The rotarod test was conducted over 2 days. On the first day, the mice were placed on a rotating rod (MED, ENV-575A, USA, the lane width was 50 mm, and the rod diameter was 30 mm) that rotated at a speed of 4 rpm for 5 min to allow the mice to learn to walk on the wheel. After the mice were trained, the speed was increased from 4 rpm to 40 rpm in even intervals within 5 min. The mice were trained three times with an interval of 30 min each time. The mice were tested the next day. Each animal underwent three trials. The length of time that the mice managed to remain on the rod and the speed at which they fell off the apparatus were recorded. The average of these measurements over three trials were used for further analysis.