The levels of interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-α) in the supernatants of rat hippocampal and cerebral cortex homogenates as well as BV-2 cells were examined using commercially available ELISA kits. The experimental procedures were conducted in accordance with the manufacturer’s instructions (Elabscience Biotech, Wuhan, China).
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