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Cell Viability Assessment
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Cell viability was determined using CCK-8 assay. The cells were plated onto 96-well plates with 4000 cells in 100 μL in each well. Then, the indicated dose of GLN was added and the cells were incubated for 24 h. Thereafter, 10 μL of CCK-8 reagent was added into each well. The plates were maintained at 37°C for 2 hours. The absorbance at a wavelength of 450 nm was quantified utilizing a microplate reader. Data are displayed as viability relative to the controls.
Copyright and License information: ©2020 Xiong et al.
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