Human aortic endothelial cell culture

YB Yvonne Baumer
AD Amit K. Dey
CG Cristhian A. Gutierrez-Huerta
NK Noor O. Khalil
YS Yusuke Sekine
GS Gregory E. Sanda
JZ Jie Zhuang
AS Ankit Saxena
ES Erin Stempinski
YE Youssef A. Elnabawi
PD Pradeep K. Dagur
QN Qimin Ng
HT Heather L. Teague
AK Andrew Keel
JR Justin A. Rodante
WB William A. Boisvert
LT Lam C. Tsoi
JG Johann E. Gudjonsson
CB Christopher K.E. Bleck
MC Marcus Y. Chen
DB David A. Bluemke
JG Joel M. Gelfand
DS Daniella M. Schwartz
HK Howard S. Kruth
TP Tiffany M. Powell-Wiley
MP Martin P. Playford
NM Nehal N. Mehta
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Human aortic endothelial cells (HAoEC) were purchased from PromoCell, Germany. HAoEC were cultured strictly following the manufacturer's recommendations using Endothelial Cell Growth Media MV2 and the PromoCell Split Kit. HAoEC were used up to passage 9. The media was supplemented with the supplement mix provided by the manufacturer, which contains 4% FBS and 2.5 μg/ml free cholesterol.

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