Human aortic endothelial cells (HAoEC) were purchased from PromoCell, Germany. HAoEC were cultured strictly following the manufacturer's recommendations using Endothelial Cell Growth Media MV2 and the PromoCell Split Kit. HAoEC were used up to passage 9. The media was supplemented with the supplement mix provided by the manufacturer, which contains 4% FBS and 2.5 μg/ml free cholesterol.
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