Mouse xenograft model

NSG mice were purchased from JAX Labs as 5 week old, females and allowed to acclimate form one week prior to the study. MOLM13 cells were injected into tail veins NSG mice (3×10⁵ cells/mouse) and allowed to engraft for 48 hours. Thereafter, mice were treated daily by oral gavage with vehicle, venetoclax (25 mg/kg), ibrutinib (25 mg/kg), or the IBR+VEN combination (5 mice/treatment group) administered sequentially; i.e., venetoclax (25 mg/kg) followed 2 hours later with ibrutinib (25 mg/kg)). On day 17 (15 days of treatment), animals were euthanized and assessed for disease burden. A sample size of 5 animals in the vehicle and 5 animals in each of the drug arms provides >90% power for a comparison of each drug vs. vehicle, and 80% power for comparison of single agent vs. combination with 5% significance level. This study was approved by the Oregon Health & Science University IACUC.