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The melan-a cells were distributed in a 48-well plate (2 × 104 cells/well) and grown in the incubator at 37°C and 10% CO2 for 24 hr. Following incubation, 500 μL of RNEE and RVEE diluted with RPMI-1640 medium to a range of concentrations (25, 50, 100, and 200 μg/mL) were added to the wells and the cells were grown in the incubator at 37°C and 10% CO2 for 72 hr, and then washed. The same treatment was repeated once more. Next, melanin was dissolved in 1 N NaOH, and the absorbance was measured at 490 nm using a plate reader.
Copyright and License information: ©2016 The Korean Society Of Toxicology
This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
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