4.4. IC50 Determination

171A4 Cells were seeded in 96-well plates and treated with 1 nM, 10 nM, 250 nM, 500 nM, 1 µM, 50 µM, 250 µM, 500 µM and 1 mM Tivantinib for 24 h in triplicate for each concentration. Following treatment, the percentage of cell survival was determined by MTT assay. The IC50 value of Tivantinib was determined by fitting a sigmoidal dose-response curve to the data, using the GraphPad Prism8. X values for concentration were transformed into scale. The Y values for MTT readouts were normalized to %. A linear regression analysis was performed using “dose response inhibition” and “log inhibitor versus normalized response”. The results were presented as IC50 and R square values.