The MEME Suite v5.0.5 Analysis of Motif Enrichment tool was utilized to determine enrichment of TF binding motifs (HOCOMOCO v11 database) in DARs, compared to shuffled input sequences (Supplementary Material, Table S5) (62,118). TF footprinting in DARs was performed utilizing RGT-HINT software (119). Similarity among HOCOMOCO v11 TF motifs was calculated using STAMP alignment software (http://www.benoslab.pitt.edu/stamp/), phylogenies were visualized with FigTree v1.4.4 (http://tree.bio.ed.ac.uk), and clades of analogous TF motifs were determined using CTree v1.03 (63,120).
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.