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Preparation of home-made mesh columns

HK Hiroyuki Kakui
ET Eriko Tsurisaki
HS Hidenori Sassa
YM Yoshinari Moriguchi
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To remove large and small debris, two types of polyester mesh [20-μm opening size (MEDIFAB 07-20/13, Sefar AG, Switzerland) and 100-μm opening size (PETEX PET105, Sefar AG)] were used. The concept of using mesh columns was derived from a protein experimental protocol [26]. The bottom of a 0.6-mL sample tube was removed around the 100-μL line using scissors and bound with the polyester mesh by heating (for details see Fig. 1). The pieces of mesh were 1 × 1 cm (Fig. 1).

Home-made mesh columns. a Left to right: 0.6-mL tube, 0.6-mL tube with cut tip, mesh attached to 0.6-mL tube (column), and polyester meshes (20-μm and 100-μm opening sizes). b–g Column preparation. The bottom of a 0.6-mL sample tube was removed around the 100-μL line using scissors (b and c). The cut surface was heated using a cigarette lighter (d and e). The heated surface was bound with 1 × 1 cm pieces of polyester mesh (f and g). The background squares were 1 × 1 cm

Copyright and License information: The Author(s) ©2020
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