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cAMP Assay

ZZ Zhichao Zhang
YL Yingfei Liu
YL Yan Luan
KZ Kun Zhu
BH Baoqi Hu
BM Bo Ma
LC Li Chen
XL Xuan Liu
HL Haixia Lu
XC Xinlin Chen
YL Yong Liu
XZ Xiaoyan Zheng
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RPCs were grown in poly-L-lysine-coated 96-well plates and cultured for 1 day. After the transfection treatment, cells were treated with 30 μM VU0155041 or 10 μM Forskolin, with the control group medium just containing the same volume of solvent. After culturing for 3 days, the concentration of intracellular cAMP was detected using an ELISA kit (R&D Systems, United States). The absorbance was detected at 450 nm using a multi-microplate spectrophotometer (BioTek). Triplicate parallel wells were carried out, and the data were obtained from the average of at least three independent experiments.

Copyright and License information:  ©2020 Zhang, Liu, Luan, Zhu, Hu, Ma, Chen, Liu, Lu, Chen, Liu and Zheng.
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