ATR-FTIR spectroscopy was used to characterize PSS layer formed. IR spectra of PSS and PSS-MNPs were recorded with a Fourier-transform infrared spectrometer (Bruker Vertex 80 v, Germany) equipped with KBr beam splitter and DTGS-detector. The spectra of 20 mg of samples were recorded from 550 to 4000 cm−1 at room temperature at a resolution of 2 cm−1. The spectrometer was calibrated by running an OPUS Validation Program, a test using software for comprehensive instrument qualification, before performing.
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