Cell Counting Kit-8 (CCK-8) assay

To measure cell viability, the transfected pancreatic cancer cells were seeded in 96-well plates (1×10³ cells/well). A total of 100 µl complete culture medium containing 10 µl CCK-8 reagent (Beyotime Institute of Biotechnology) was added to each well at different time points of interest. After incubation in the dark at 37°C for 2 h, the absorption at 450 nm was detected. Each group had five duplicate wells and the experiments were repeated in triplicate.