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MiRNA overexpression was achieved by transfection with human miR-302b precursor or negative control (Thermo Fisher Scientific) and verified by qRT-PCR. For gene knockdown, specific siRNAs, si-ITGA6, si-E2F1, or their negative control (Thermo Fisher Scientific) were used. Cells were transfected with 100 nmol/L miRNA precursor or siRNAs and their negative controls complexed with Lipofectamine 2000 reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions.
Copyright and License information: ©2020 by the authors.
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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