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For the evaluation of ALDH1A1 activity, an ALDEFLUOR kit (STEMCELL Technologies, Vancouver, BC, Canada) was used according to the manufacturer’s instructions. Briefly, siCONT/NEDD4-treated cells were cultured in growth media supplemented with 10 μl ALDEFLUOR reagent for 30 min, and then green fluorescence was detected by microscopy (Revolve, ECHO, San Diego, CA, United States).
Copyright and License information: ©2020 Jeon, Kim, Lee and Cho.
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