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The size and polydispersity index (PDI) of the LNPs were measured using dynamic light scattering in 1× PBS (ZetaPALS, Brookhaven Instruments; Table 2). Zeta potentials were measured using the same instrument in a 0.1× PBS solution. Diameters are reported as the largest intensity mean peak average, which constitutes >95% of the nanoparticles present in the sample. To calculate the nucleic acid encapsulation efficiency, a modified Quant-iT RiboGreen RNA assay (Invitrogen) was used as previously described.53
Characterization of LNP Formulations Used in the Manuscript
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