4.3. Injection of Aβ25–35

For aggregation of Aβ_25–35, Aβ_25–35 was dissolved in saline solution and it was incubated at 37 °C for 3 days. Aggregated Aβ_25–35 solution was intracerebroventricularly (i.c.v.) injected to mice in accordance with procedure established by Laursen and Belknap [48]. The mice were anesthetized with a mixture of zoletil50 and rompun (3:1 ratio) by intraperitoneal injection. After anesthetization of mouse, aggregated Aβ_25–35 (25 nM/5 μL) was injected in the bregma of mouse into the lateral ventricle using a microinfusion pump (Baoding Longer Precision Pump Co., Ltd., Baoding, China) at the following coordinates: anterior/posterior −0.8 mm, medial/lateral −1.5 mm, and dorsal/ventral −2.2 mm [20].