MTT Assay for Determination of Macrophage Viability

YS Yulong Shi
PL Peng Luo
WW Weikang Wang
KH Klemens Horst
FB Felix Bläsius
BR Borna Relja
DX Ding Xu
FH Frank Hildebrand
JG Johannes Greven
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Cell viability was assessed using an MTT assay. Briefly, 0.1 volume of MTT (1 mg/ml) was added to the cell culture medium and incubated for 4 h. The medium was removed, and 100 μl of DMSO were added into each well, and the plate was gently rotated to completely dissolve the precipitation. The absorbance of MTT was measured at 450/620 nm using a BioTek Synergy (BioTek, Winooski, VT, USA).

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