Cell viability was assessed using an MTT assay. Briefly, 0.1 volume of MTT (1 mg/ml) was added to the cell culture medium and incubated for 4 h. The medium was removed, and 100 μl of DMSO were added into each well, and the plate was gently rotated to completely dissolve the precipitation. The absorbance of MTT was measured at 450/620 nm using a BioTek Synergy (BioTek, Winooski, VT, USA).
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