Cell Lines and Culture Conditions

Rajashekhar Kanchanapally; Mohammad Aslam Khan; Sachin Kumar Deshmukh; Sanjeev Kumar Srivastava; Moh’d Khushman

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Cell Lines and Culture Conditions

RK Rajashekhar Kanchanapally

MK Mohammad Aslam Khan

SD Sachin Kumar Deshmukh

SS Sanjeev Kumar Srivastava

MK Moh’d Khushman

This method is extracted from research article: ACS Omega, Aug 2020

Exosomal Formulation Escalates Cellular Uptake of Honokiol Leading to the Enhancement of Its Antitumor Efficacy

DOI: 10.1021/acsomega.0c03136

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Pancreatic cancer cell lines (MiaPaCa and Colo357), breast cancer cell line (MDA-MB-231), colon cancer cell line (HT-29), prostate tumor cell line (LNCaP), and ovarian cancer cell line (SKOV-3) were procured, maintained, and authenticated as previously described.³⁶⁻⁴⁰ Mesenchymal stem cells (MSCs) were procured from American Type Culture Collection (Manassas, VA) and maintained in special mesenchymal stem cell basal media that was supplemented with growth factors. All the cells were routinely tested for mycoplasma contamination in our in-house facility.

This is an open access article published under an ACS AuthorChoice License, which permits copying and redistribution of the article or any adaptations for non-commercial purposes.

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