2.4. Microtubule Regrowth Assay

Cells growing on glass coverslips were treated with nocodazole (150 ng/mL) for 16 h at 37 °C. The cells were then fixed with 4% paraformaldehyde for 15 min. at room temperature 1 h after the removal of the nocodazole. The cells were subjected to immunofluorescence assay using a tubulin antibody (sc-5286, Santa Cruz Biotechnology, Santa Cruz, CA, USA) followed by Alexa Fluor Plus 555 secondary antibody (orange) (A32727, Thermo Fisher Scientific, Waltham, MA, USA).