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Total RNA was extracted from prostate cancer cells using Trizol reagent (Invitrogen, United States), followed by cDNA preparation using a reverse transcription kit (Thermo, United States). The cDNAs were amplified by qRT-PCR using SYBR Green PCR Master Mix (Roche, United States) on a LightCycler480 system, and relative abundance was determined using the ΔCt method.
The PCR primers were shown in Table 2.
Oligonucleotide sequence of primer set used to amplify in each cDNA.
Copyright and License information: ©2020 Wang, Wang, Zhang, Meng, Chen, Wang, Jiang and Ma.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
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