Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Request a Protocol
Ask a
question
Favorite
Tissue sections obtained from the brains of 72-h post-injury mice were used to examine cell death with an In-Situ Cell Death Detection Kit, as previously described (Cuello-Carrión and Ciocca, 1999). After being warmed to RT for 30 min, the sections were fixed in acetone for 8 min at 4°C. After being rinsed with PBS, the sections were treated with 3% BSA for 30 min at 37°C before being incubated with TUNEL reaction mixture in the dark for 90 min at 37°C. The nuclei were counterstained with DAPI for 5 min.
Copyright and License information: ©2017 Sun, Gu, Wang, Chai, Fan, Yang, Xu, Gao, Li, Yin, Zhou, Chen and Zhang.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
