Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Ask a
question
Favorite
Anti‐CD3/CD28‐activated purified CD3+ T cells were stained with a CFSE Cell Division Tracking Kit (BioLegend, San Diego, CA, USA), and they were exposed to different groups of CMs for 5 days. On day 5, the cells were harvested, and the percentage of proliferating cells was assessed by CFSE fluorescent staining and analysed by flow cytometry.
Copyright and License information: ©2020 The Authors. published by John Wiley & Sons Australia, Ltd on behalf of Australian and New Zealand Society for Immunology, Inc.
This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
