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Measurement of glucose uptake and lactate production
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The glucose uptake assay was performed as previously published. Briefly, cells that received the indicated treatments were plated in 6‐well plates, and then, 10 μM 2‐NBDG (Invitrogen) was added to the medium for 2 h, when the cells were 80% confluent. After incubation, flow cytometry was performed to detect the percentage of glucose uptake. Lactate production, ATP concentration, and HK2 activity were determined with specific kits (BioVision) according to the manufacturer's instructions.
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This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
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