2.21. Enzyme-linked immunosorbent assay (ELISA) for IL-6 and TNF-α measurements in brain

After MWM behavioral test, APP/PS1 mice were sacrificed. Brain tissues were lysed in buffer containing 50 mM Tris (pH 7.4), 150 mM NaCl, 1% Triton X-100, 1% sodium deoxycholate, 0.1% SDS and protease inhibitors. The homogenate was sonicated and centrifuged (12,000 RPM, 20 min, 4 °C), and the supernatant was kept for further analysis. Collected samples were respectively measured via Mouse IL-6 ELISA Kit and Mouse TNF-α ELISA Kit (Beyotime Biotechnology Co., Ltd., China) according to the manufacturer's instructions.