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4.7. Anchorage-Independent Growth by Soft Agar Assay
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Two thousand transfected NIH3T3 cells in 2 mL of cell culture medium containing 10% FCS with 0.3% agar were laid on a 1-ml basal layer of 0.8% wt/v agar in 35 mm plates. Fresh medium was added every week. Colony formation was determined on day 21. Three repeats were done for each transformant.
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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