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U-251 MG cell culture

MD Matthew Drill
KP Kim L. Powell
LK Liyen Katrina Kan
NJ Nigel C. Jones
TO Terence J. O’Brien
JH John A. Hamilton
MM Mastura Monif
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The U-251 MG human glioblastoma cell line (U251; Sigma) was maintained in Dulbecco’s modified Eagle’s Medium (DMEM; Lonza), supplemented with 10% heat-inactivated Fetal Bovine Serum (FBS; Life Technologies), penicillin–streptomycin (5,000 U/mL; Life Technologies), 1% non-essential amino acids (Sigma) and sodium pyruvate (1 mM; Sigma). Cells were cultured in a humidified incubator at 37 °C, 5% CO2/95% O2 and routinely passaged at 80% confluency.

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