Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Monoclonal antibody (mAb) and TRAP 5a separation from TRAP 5b

CP Christina Patlaka
BT Bojan Tubic
PL Pernilla Lång
SP Staffan Paulie
DS Diana Swolin-Eide
PM Per Magnusson
GA Göran Andersson
request Request a Protocol
ask Ask a question
Favorite

The mouse monoclonal antibody 46 (Mabtech) recognizes TRAP 5a [12] and, thus, plates coated with mAb 46 were used for quantifying and clearing of TRAP 5a in serum samples. MAb 25.44 (Mabtech) recognizes both TRAP 5a and TRAP 5b; thus, it was used after pre-incubation of samples with mAb 46 to capture and quantify the remaining fraction of TRAP that should only contain TRAP 5b.

Copyright and License information: The Author(s) ©2019
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

0/150

tip Tips for asking effective questions

+ Description

Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.

post Post a Question
0 Q&A