2.1. Plant materials

SA Sahar Abdelaziz
HY Hanan M. Al Yousef
AA Ali S. Al-Qahtani
WH Wafaa H.B. Hassan
OF Omer I. Fantoukh
ME May A. El-Sayed
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The aerial parts of P. aculeata L. were collected in 2013 from the Diriyah region, Riyadh city, Saudi Arabia. The plant was verified by Prof Dr. Jakob Thomas; College of Science, KSU and Prof Dr. Husain Abdel Basset, professor of Taxonomy, Faculty of Science, Zagazig University, Egypt. A voucher specimen (#PA-513) was prepared and deposited at the herbarium in the Department of Pharmacognosy, Faculty of Pharmacy, Zagazig University. P. aculeata aerial parts were air-dried and ground into coarse particles until use.

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