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Cell viability assay
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The in vitro cell viability was determined by MTT assay. The cells (5 × 104 cells/ml) were seeded into 96-well culture plates. After overnight incubation, the cells were treated with various concentrations of agents for 48 h. The optical density of each well was measured at 570 nm with a Molecular Devices M5 Reader. The experiments were performed in triplicate, and results were plotted as the mean ± s.e.m.
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