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Annexin V/PI Staining-Based Apoptosis Assay

YL Yusi Liu
XS Xue Song
MW Moli Wu
JW Jiao Wu
JL Jia Liu
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The Annexin V-FITC Apoptosis Detection Kit (Nanjing Key Gen Biotech Co., Nanjing, China) was used for quantification of apoptosis. Cells (2 × 105/well) were seeded into 6-well plates and were collected after 48 h drug treatments. Externalized phosphatidylserine was labeled with annexin-V-FITC-conjugated for 15 min on ice. Propidium iodide (PI, 1 μg/mL) was added 10 min prior to FACS analysis. Viable (annexin-/PI-), early (annexin+/PI-) and late apoptotic (annexin+/PI+) and necrotic cells (annexin-/PI+) were assigned. The labeled cells were identified by flow cytometry BD Accuri C6 (BD Biosciences, San Jose, CA, USA).

Copyright and License information:  ©2020 Liu et al.
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