Electroretinogram Recording to Assess The Visual Function of Rabbits

Scotopic flash ERG recording was performed at 3mo post-operation as described previously[16]. Briefly, after 12-h dark adaptation, the rabbits were carefully anesthetized with 3% pentobarbital sodium (1 mL/kg). The pupils were dilated with 1% tropicamide. The body temperature was maintained at 37°C with a heating pad to prevent hypothermia. Active gold electrodes were placed onto cornea serving as the recording electrodes. The reference and ground electrodes were placed subcutaneously in the mid-frontal areas of the head and back, respectively. We performed light stimulation at densities of -2.5, -0.5, -0.02, and 0.5 log (cd·s/m²) for New Zealand white rabbits and 0.5 log (cd·s/m²) for rabbits. The amplitudes and peak times of the a-waves and b-waves were recorded and processed through a RETI-Port device (Roland Consult). All experimental procedures were performed in a dark room under dim red safety light.