To induce muscle regeneration, 100 μl of 10 mM cardiotoxin (CTX; C9759, Sigma-Aldrich) were injected intramuscularly into the tibialis anterior (TA) muscle of anesthetized 10-week-old male mice using a 29 G syringe. Regenerating muscles were isolated 2, 4 and 7 days after CTX injection.
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